这是所有仙女狂热者的公共服务公告。如您所知,我们正在对属进行粗略的系统发育评论Cirrhilabrus在其所有亲密和巴洛克式的细节中。在比较形态学数据的过程中,由于这种限制,我们无法完全理解该属。遗传物质的分子分析无疑将帮助我们揭示围绕更棘手的群体的一些奥秘。这就是你come in!
由于该属太大,所以我们少数人几乎不可能收集每个物种的遗传物质。这就是为什么我们需要您的帮助。考虑一下这是招聘驱动器。加利福尼亚科学院的路易斯·罗沙(Luiz Rocha)博士需要样本 - 尽可能多的物种。我们拥有的物种越多,我们就越能够对其进行顺序和比较。这不可避免地会帮助我们在当前的系统发育综述中确认我们的一些假设。
全部we need from you is a little clip of your fish. Any part of the fin would work, but we find working with the caudal fin the easiest and most convenient. Your fish will regenerate its fin in a couple of days.
Here’s what you need to do:
1)轻轻摇动您的手掌。
2) With a pair of clean and sharp scissors, gently and swiftly snip a small portion of the fin. Be sure to cut only the soft portions of the fin. Severing the spinous rays on the anterior dorsal fin for example will not result in proper and full regeneration. The caudal fin and the posterior soft dorsal fin are the best places to obtain fin clippings. A small clip of <1 cm is sufficient.
3) Quickly place the severed fin portion in a clean vial of 75-90% ethanol (ethyl alcohol). It is important to ensure that the concentration of alcohol is within this range, and ensure that it is lab grade.不要use isopropyl alcohol (rubbing alcohol).不要将您的标本保留在福尔马林中,因为这将使DNA变色,从而使其无用。*您可能需要接触科学机构或实验室才能获得酒精。
4)尽可能准确地标记样品。Species name, size, date of preservation, location of collection (if you know) are very important documentations. Make sure that each vial contains only one specimen, so as to avoid mix ups and confusion.
5) Bring them over to MACNA!
* We understand that identifying fairy wrasses can be tricky. We strongly advice you to take a photo of your fish that you are sampling as well. In the event where you are unable to identify it, label your sample vial with a number corresponding to the photo of the same fish. Label the photo with the same number as well, and show this to us at MACNA or email it to us.
全部Cirrhilabrus欢迎鳍片剪辑,但这里有更急需的标本清单:
–Cirrhilabrus brunneus
–Cirrhilabrus lunatus
–Cirrhilabrus cf.Lunatus
–Cirrhilabrus sp.“ Pintail”
–Cirrhilabrus Claire
–cirrhilabrus pylei
–Cirrhilabrus cf.pylei
–Cirrhilabrus katoi
–cirrhilabrus luteovittatus
–Cirrhilabrus solorensis
–Cirrhilabrus sp.“Bali red head”
–Cirrhilabrus scottorum(all regional forms)
–cirrhilabrus大优势(all regional forms)
–Cirrhilabrus rubrimarginatus(all regional forms)
更好的是,如果您的冰柜中有任何已故的标本,或者从那时起的机会和新鲜死亡,则do not throw them away。只需将整个鱼粘在足够的乙醇中,然后标记样品即可。
Come on up to the reef builders booth at MACNA and drop your fin clippings into our “DNA Box”. Citizen scientists like you can help us better understand these fish that we’ve come to know and love.